ENERGY METABOLISM IN SKELETAL MUSCLES IN DOGS DURING TREATMENT OF SHIN FRACTURES WITH ILIZAROV’S TECHNIGUE

Aim - to study the dynamics of the recovery of energy resources in the tibialis anterior (TA) in dogs after shin fractures. Material and methods. The concentration of energy metabolism substrates, the rate of glycolysis and glycogenolysis in TA in 25 dogs during the treatment of tibial fractures by Ilizarov were studied. The values parameters in the experimental animals were compared with values in the intact (non-operated) dogs. Results. The level of glycogen and glucose decreased in TA within 14 days of fixation. On the 28th day of fixing the level of these metabolites corresponded to the level in intact animals. By the end of fixation the rate of glycogen utilization in TA of experimental animals was significantly reduced relative to intact animals, and the rate of glucose utilization was unchanged, though all glucose was almost entirely utilized by aerobic way. A month after treatment there was an increase of glycogen levels in the TA and activity of lactatedehydrogenase MM-isoenzyme. When glycogen solution added to the cell-free culture of muscle the its considerable utilization and the pyruvate accumulation were found. Conclusions. Anaerobic energy metabolism of in the TA of injured segment have prevailed before the 14-th day after the injury, the aerobic energy metabolism - after 28-days. The phenomenon of growth of anaerobic energy metabolism in TA of injured segment within three months after treatment was revealed.

перелом, скелетные мышцы, углеводный обмен, fracture, skeletal muscle, carbohydrate metabolism