Coatings Based on Two-Dimensionally Ordered Linear Chain Carbon for Protection of Titanium Implants from Microbial Colonization

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Abstract

Purpose of the study – to evaluate the antibacterial activity and biological compatibility of alloy coatings based on two-dimensionally ordered linear chain carbon (TDOLCC).

Materials and Methods. Coatings based on TDOLCC were synthesized using alloying additions like nitrogen (TDOLCC+N) and silver (TDOLCC+Ag) on the surfaces of titanium plates and polystyrene plates by the ion-stimulated carbon condensation in a vacuum. The authors examined the superficial bactericidal activity of the coatings and its resistance to mechanical effects. Coated plates were evaluated in respect of rate of microbial biofilms formation by clinical isolates with multiple and extreme antibiotic resistance. Specimens were colored with crystal violet solution to visualize the biofilms. Cytotoxic effect of coatings was evaluated in respect of primary culture of fibroblasts and keratinocyte cell line HaCaT.

Results. The authors observed pronounced superficial bactericidal effect of TDOLCC+Ag coating in respect of microorganisms of several taxonomic groups independently of their resistance to antibacterial drugs. TDOLCC+Ag coating proved capable to completely prevent microbial biofilm formation by antibiotic resistant clinical isolates of S. aureus and P. aeruginosa. Silvercontaining coating demonstrated mechanical resistance and preservation of close to baseline level of superficial bactericidal activity even after lengthy abrasion treatment. TDOLCC based coatings did not cause any cytotoxic effects. Structure of monolayers formed in cavities coated by TDOLCC+N and TDOLCC+Ag was indistinguishable from the monolayers in cavities of control plates.

About the authors

D. V. Tapalski

Gomel State Medical University

Author for correspondence.
Email: tapalskiy@gsmu.by

Dmitry V. Tapalski — cand. Sci. (Med.), associate professor, head of the Department of Microbiology, Virology and Immunology

Gomel

Belarus

N. S. Nikolaev

Federal Center of Traumatology, Orthopedics and Endoprosthetics, Cheboksary

Email: fake@neicon.ru

Nikolai  S.  Nikolaev —  Dr.  Sci.  (Med.),  head  physician

Cheboksary

Russian Federation

A. V. Ovsyankin

Federal Center of Traumatology, Orthopedics and Endoprosthetics, Smolensk

Email: fake@neicon.ru

Anatoly V. Ovsyankin — cand. Sci. (Med.), head physician

Smolensk

Russian Federation

V. D. Kochakov

Ulyanov Chuvash State University

Email: fake@neicon.ru

Valery D. Kochakov — cand. Sci. (eng.), professor at the Department of Applied Physics and Nanotechnologies

Cheboksary

Russian Federation

E. A. Golovina

Federal Center of Traumatology, Orthopedics and Endoprosthetics, Smolensk

Email: fake@neicon.ru

Elena A. Golovina — clinical bacteriologist

Smolensk

Russian Federation

M. V. Matveenkov

Institute of Radiobiology of the National Academy of Sciences of Belarus

Email: fake@neicon.ru

Matvei V. Matveenkov — researcher, Combined Effects Laboratory

Gomel

Belarus

M. V. Sukhorukova

Research Institute of Antimicrobial Chemotherapy of Smolensk State Medical University

Email: fake@neicon.ru

Marina V. Sukhorukova — cand. Med. Sci., senior researcher, head of the Department of Multicenter Study 

Smolensk

Russian Federation

R. S. Kozlov

Research Institute of Antimicrobial Chemotherapy of Smolensk State Medical University

Email: fake@neicon.ru

Roman S. Kozlov — Dr. Sci. (Med.), professor, corresponding member of the Russian Academy of Sciences; director of Research Institute of Antimicrobial Chemotherapy of Smolensk State Medical University

Smolensk

Russian Federation

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