Traumatology and Orthopedics of RussiaTraumatology and Orthopedics of RussiaТравматология и ортопедия России2311-29052542-0933Vreden National Medical Research Center of Traumatology and Orthopedics8410.21823/2311-2905-2014-0-3-104-114METHODS OF EXAMINATIONSМЕТОДЫ ИССЛЕДОВАНИЙPCR IN TRAUMATOLOGY AND ORTHOPAEDICS: METHOD DESCRIPTION AND APPLICABILITYПЦР В ОБЛАСТИ ТРАВМАТОЛОГИИ И ОРТОПЕДИИ: СУТЬ МЕТОДА И ВОЗМОЖНОСТИ ПРИМЕНЕНИЯPolyakovaE. M.ПоляковаЕ. М.
Russian Federation
ekaterinapolyakova@rambler.ruBozhkovaS. A.БожковаС. А.
Russian Federation
clinpharm-rniito@yandex.ruVreden Russian Research Institute of Traumatology and OrthopaedicsФГБУ «Российский научно-исследовательский институт травматологии и ортопедии им. Р.Р. Вредена» Минздрава России1210201420310411412092016https://journal.rniito.org/jour/article/view/84

Review brief presents description of polymerase chain reaction method (PCR) and its most common variants. Three PCR-based lines of research, carried out in the traumatology and orthopaedics, include identifying a causative agents of the implant-associated infection after orthopaedic surgery; detection of antibiotic resistance genes and biofilm forming genes. It was shown that PCR can be used as additional method for detection of genetic disorders, significant for traumatology and orthopaedics, and for investigation of cartilage and bone regeneration.

В обзоре кратко изложены сущность метода полимеразной цепной реакции и его наиболее распространенные варианты. Выделены три основных направления исследований в области травматологии и ортопедии, в основе которых лежит метод полимеразной цепной реакции: выявление возбудителей имплантат-ассоциированной инфекции; выявление генов антибиотикорезистентности и генов биопленкообразования микробных возбудителей. Приведены примеры применения полимеразной цепной реакции для выявления значимых в травматологии и ортопедии наследственных заболеваний, а также в качестве дополнительного метода при исследованиях, связанных с процессами регенерации хрящевой и костной тканей.

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