Traumatology and Orthopedics of RussiaTraumatology and Orthopedics of Russia2311-29052542-0933Vreden National Medical Research Center of Traumatology and Orthopedics201610.17816/2311-2905-2016Research ArticleDeproteinized Bone Tissue as a Matrix for Tissue-Engineered Construction: Experimental StudyAnastasievaEvgeniya A.evgeniya.anastasieva@gmail.comhttps://orcid.org/0000-0002-9329-8373CherdantsevaLiliya A.<p>Cand. Sci. (Med.)</p>cherdanceff@yandex.ruhttps://orcid.org/0000-0002-4729-3694TolstikovaTatyana G.<p>Dr. Sci. (Biol.)</p>tg_tolstikova@mail.ruhttps://orcid.org/0000-0002-3750-2958KirilovaIrina A.<p>Dr. Sci. (Med.)</p>irinakirilova71@mail.ruhttps://orcid.org/0000-0003-1911-9741Novosibirsk Research Institute of Traumatology and Orthopedics n.a. Ya.L. TsivyanN.N. Vorozhtsov Novosibirsk Institute of Organic Chemistry of Siberian Branch of Russian Academy of Sciences1104202329146592710202226122022Copyright © 2023, Eco-Vector2023<p><strong><em>Background. </em></strong>At present, for a number of reasons the complete bone defect replacement with autogenous bone is not always possible. Bone substitute materials are used as an alternative to autogenous bone tissue and can be of either biological or non-biological origin. One of the ways of development of reconstructive technologies is the use of tissue-engineered constructs that fully imitate autogenous bone tissue in the required volume.</p>
<p><strong><em>Aim of study </em></strong> to define in vivo the possibility of using deproteinized human cancellous bone tissue as a matrix for creating tissue-engineered constructs.</p>
<p><strong><em>Methods. </em></strong>An in vivo study was carried out on NZW rabbits. To create a construct, we used the fragments of deproteinized cancellous bone tissue of the human femoral head and stromal vascular fraction of rabbit adipose tissue as a matrix. Bone defect modeling with its subsequent replacement was performed to evaluate the efficacy of reparative osteogenesis during bone defects reconstruction. Study groups were defined: group 1 (control) surgical modeling of a bone defect of the femur without its reconstruction; group 2 surgical modeling of a bone defect of the femur with its reconstruction using fragments of deproteinized cancellous bone matrix; group 3 surgical modeling of a bone defect of the femur with its reconstruction using fragments of deproteinized cancellous bone matrix in combination with stromal vascular fraction of adipose tissue (according to ACP SVF technology).</p>
<p><strong><em>Results. </em></strong>Comparative analysis of reparative processes in case of applying tissue-engineered constructs based on deproteinized human cancellous bone matrix in combination with adipose tissue-derived stromal vascular fraction on in vivo experimental model revealed that the use of these bone substitute materials contributes not only to an early activation of reparative regeneration of main structural elements of the bone tissue in the area of the bone defect replacement, but also to its well-timed differentiation. This determines the restoration of structural and functional viability of the bone tissue at the damage site without developing discernible reactive inflammation. Moreover, the effect of the selected tissue-engineered construct with the combined influence of several factors (ACP SVF) in its composition turned out to be more effective in stimulating bone tissue repair and differentiation.</p>
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